Journal:
Article Title: Distinct Roles for the AAA ATPases NSF and p97 in the Secretory Pathway
doi: 10.1091/mbc.E03-02-0097
Figure Lengend Snippet: NSF(E329Q) disrupts Golgi function, whereas p97(E578Q) does not. (A) Synthesis of 35S-sulfated GAGs as a function of the time of NSF(E329Q)myc induction. Data for the different time points were normalized relative to 100% for the 0 time point. (B) Secretion of 35S-sulfated GAGs relative to the total 35S-sulfated GAGs synthesized for different chase times in p97(E578Q)myc cells that were induced for 12 h (ON) or were not induced (OFF). (C) VSV-G ts045-GFP distribution in p97myc cells (top panel) and p97(E578Q)myc cells induced for 12 h (bottom panel) at 0, 30, 60, and 120 min after shifting the cells from the nonpermissive temperature (38.5°C) to the permissive temperature (32°C). These images were obtained on a confocal microscope. Each box is 92 × 92 μm.
Article Snippet: p97(E578Q) and U2OS cells were transfected with VSV-G ts045-GFP ( Presley et al., 1997 ) using Lipofectamine (Invitrogen) according to the manufacturer's instructions.
Techniques: Synthesized, Microscopy